Coding

Part:BBa_K5226045:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-22)


fmo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 484
    Illegal AgeI site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Synthetic（BGI）. Sequence source is from Methylophaga aminisulfidivorans, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/AF494423）

References