Coding

Part:BBa_K5226016:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-29)


Vib-sdaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 483bp base pair of Vib-sdaA from g/c to a/t.

Source

Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1660693&to=1662072）

References