Coding

Part:BBa_K5226016

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-29)

Vib-sdaA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 919
    Illegal PstI site found at 488
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens.

Usage and Biology

Vib-SdaA is serine dehydratase. The protein sequence is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the conversion of L-serine into pyruvate during the glycine assimilation(C3M).


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Parameters
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