Coding

Part:BBa_K5226013:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-29)


Vib-gcvH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation.. Mutate the 243bp base pair of Vib-gcvH from g/c to a/t.

Source

Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1375221&to=1375601）

References