Coding

Part:BBa_K5226004

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-27)


TD-gcvT

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

TD-GcvT is Aminomethyltransferase.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the conversion of S-aminomethyl-dihydrolipoyllysine into dihydrolipoyllysine during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 627
    Illegal PstI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None