Coding

Part:BBa_K5226000:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-06)


AM1-ftfl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 379
    Illegal AgeI site found at 693
    Illegal AgeI site found at 721
    Illegal AgeI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 678bp, 1320bp and 1326bp of AM1-ftfl from g/c to a/t.

Source

Synthetic（BGI）. Sequence source is from Methylobacterium extorquens AM1, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP001510.1?from=340743&to=342416）

References