Part:BBa_K5226000

AM1-ftfl

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 379
  Illegal AgeI site found at 693
  Illegal AgeI site found at 721
  Illegal AgeI site found at 859
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

AM1-Ftfl is Formate-tetrahydrofolate ligase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of formate into 10-formyltetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) .