Coding

Part:BBa_K5223000

Designed by: Chong Yao   Group: iGEM24_SCU-China   (2024-09-30)


GjUGT94E13_opt (UDP-glucosyltransferase)

The protein encoded by the GjUGT94E13 gene is a UDP-glycosyltransferase that participates in crocin biosynthesis in Gardenia jasminoides. In previous studies, GjUGT94E13 was shown to be responsible for catalyzing the production of crocetin from crocetin by adding glucose molecules to the crocetin molecule.

Biology and Usage

UDP-glucosyltransferase
Function Crocin synthesis
Use in Nicotiana benthamiana
Backbone pZYB11-pEAQ-HT-DEST1
Derived from Gardenia jasminoides

Reference

[1] Xie L, Luo Z, Jia X, Mo C, Huang X, Suo Y, Cui S, Zang Y, Liao J, Ma X. Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana. International Journal of Molecular Sciences. 2023; 24(18):14139. https://doi.org/10.3390/ijms241814139

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None