Coding

Part:BBa_K5222010:Design

Designed by: Maria Avtodeeva   Group: iGEM24_Sorbonne-U-Paris   (2024-10-02)


CDS of HsOMT adapted for B3-B5 position of MoClo ( codon optimized for chlamydomonas reinhardtii)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 37
    Illegal XhoI site found at 90
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our CDS needs to be adapted for MoClo assembly, so we plan to insert our gene into positions B3-B5 of the MoClo system. To do this, we added appropriate fusion sites at the ends of the sequence. At the 5' end, we incorporated a B3 scar (AATG) along with the BbsI restriction site, and at the 3' end, a B5 scar with a BbsI restriction site Additionally, to prevent any internal cleavage of our gene during the digestion/ligation process, we removed internal type IIS restriction sites (BsaI, BbsI, and SapI).


Source

article Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET Kim, Hee & Kim, Jae & Cha, Hyun & Kang, myung Jong & Lee, Hyun & Khang, Tae & Yun, Eun & Lee, Dae-Hee & Song, Bong & Park, Si Jae & Joo, Jeong Chan & Kim, Kyoung. (2019). Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET. ACS Sustainable Chemistry & Engineering. XXXX. 10.1021/acssuschemeng.9b03908.

References