Part:BBa_K5211023
Ptet-T1AB2
-Usage and Biology
Description:
The Ptet promoter was amplified by overlap PCR with the T1AB' operon to obtain the Ptet-T1AB' operon. （tesB derived from the E. coli K12 and phaA, phaB derived from C. necator H16
Usage:
BBa_K5211023 (Fig.1-1) consists of an operon driven by an inducible promoter Ptet. It was employed to enhance the expression of the synthetic operon containing the tesB,phaA, and phaB genes involved in the 3HB biosynthesis pathway. The composite part was cloned into the shuttle vector pEZ15Asp generating the plasmid, and then transformed into the recombinant ZMNP to generate the recombinant strain ZMNP-PtT1.Finally we test the fermentation performance of the strain to validate our improvements.
Fig.1-1 Feature of the composite part of BBa_K5211023 .
The fermentation performance of the recombinant strain ZMNP-PtT1 is shown in Fig.1-2. ZMNP-PtT1 reached a maximum 3HBl titer of 425.67±2.89 mg/L. This indicated that that our improvements can enhance the yield of 3HB.
Fig.1-2 Cell growth and 3HB production of ZMNP-PtT1 with 1.2 μg/mL tetracycline concentrations as an inducer.
Metabolic pathway of zymomonas mobilis
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