Part:BBa_K5211001
The gfp gene is used to encode sfGFP (Superfolder Green Fluorescent Protein), a fusion protein that
The gfp gene is used to encode sfGFP (Superfolder Green Fluorescent Protein), a fusion protein that was engineered through genetic modification of the natural GFP. It not only inherits the characteristic of emitting bright green fluorescence from GFP, but also achieves significant improvements in folding efficiency, stability, and resistance to loss. What's more important is that sfGFP has excellent promoting protein fusion function, which can efficiently fuse with the target protein to form stable fusion proteins. This fusion not only enhances the solubility and stability of the target protein, but also simplifies the protein separation and purification process. sfGFP can be highly expressed in rapidly reproducing bacteria to form stable fluorescent structures, making it easy to track and observe the fusion protein within cells. At the same time, sfGFP maintains its fluorescence activity under high temperature and acidic or alkaline conditions, making it suitable for long-term observation experiments. This characteristic makes sfGFP have extensive application value in fields such as cell marking, gene expression monitoring, and protein interaction research. By introducing the sfGFP tag, scientists can more conveniently study the functions, interactions, and dynamic changes of proteins in cells. This improved fluorescent protein provides a powerful tool for revealing cell structures, functions, and protein functions, promoting the in-depth development of life science research. With the continuous progress of biotechnology, sfGFP is expected to play an important role in more areas of life science, opening new paths for scientific exploration.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |