Part:BBa_K5210019

ProU-ScrR-Pscr-lacl-Plac-MazF

This composite part was controlled by sucrose. The sucrose can inhibits the deterrence of Pscr by ScrR, which led to the expression of lacI and the non-expression of MazF. When the sucrose concentration was reduced, the inhibition of Pscr by ScrR was enhanced, the expression of lacI was reduced, and MazF was then expressed.

Usage and Biology

This composite part enables the engineered bacteria to activate the sensing of sucrose only in the hypertonic environment of the ocean. After entering the ocean, the spare sucrose in the product and the sucrose produced by the algae inhibited the deterrence of Pscr by ScrR, which led to the expression of lacI and the non-expression of MazF. After the engineered bacteria were detached from the system, the sucrose concentration was reduced, the inhibition of Pscr by ScrR was enhanced, the expression of lacI was reduced, and MazF was then expressed to play a killing role.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 926
  Illegal BglII site found at 2659
  Illegal BamHI site found at 1261
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]