Part:BBa_K5210014
Pompc
This promoter comes from E.coli and is involved in osmotic regulation. In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane, phosphorylates the transcription factor OmpR[1]. Phosphorylated OmpR binds the ompC promoter and activates the transcription[2].
Usage and Biology
In our luminescence circuit, Pompc acts as the promoter sensing the high osmolarity in the sea.In case of high osmolarity, the protein kinase phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the PompC promoter and activates the transcription of LuxCDEFG downstream of the pOmpc promoter.This Enyz-OmpR two component system provides substrate LuxCDEFG for luxAB oxidative luminescence. We use this part for the composite part BBa_K5210017.
Experiment results
We characterized the performance of this promoter in terms of reporter gene expression.
A.plasmid construction;
B.pre-experiment to explore the suitable growth concentration of experimental bacterium BL21;
C. fluorescence intensity of engineered bacteria under different osmotic pressures;
D. Fluorescence microscope to observe the fluorescence intensity of engineered bacteria under different osmotic pressures.
Reference
[1]Cai, Sheng Jian, and Masayori Inouye. “EnvZ-OmpR interaction and osmoregulation in Escherichia coli.” The Journal of biological chemistry vol. 277,27 (2002): 24155-61. doi:10.1074/jbc.M110715200
[2]Kenney LJ, Anand GS. EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically. EcoSal Plus. 2020 Jan;9(1):10.1128/ecosalplus.ESP-0001-2019. doi: 10.1128/ecosalplus.ESP-0001-2019
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2
- 1000COMPATIBLE WITH RFC[1000]
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