Coding

Part:BBa_K5210010

Designed by: yitian zhou   Group: iGEM24_NMU-China   (2024-09-30)


Gas Vesicle Protein (GVP)

Gas vesicles are intracellular, protein-coated, and hollow organelles found in cyanobacteria and halophilic archaea. They are permeable to ambient gases by diffusion and provide buoyancy, enabling cells to move upwards in liquid to access oxygen and/or light.[1].

Usage and Biology

In our Filter membrane wrapped-floating module, gvp is controlled by constitutive promoter, which is constitutively expressed in the bacteria.Constitutively expressed gas vesicle proteins cause the bacteria to float on the water surface, reducing stacking and increasing the luminescent area.



Reference

[1]Li, N. & Cannon, M. C., Gas vesicle genes identified in Bacillus megaterium and functional expression in Escherichia coli. J BACTERIOL 180 2450 (1998).



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 487
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 487
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 487
    Illegal NgoMIV site found at 897
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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