Part:BBa_K5210006

LuxF

LuxF derives from Photorhabdus like Vibrio natriegens.It codes the protein LuxF, acting as a scavenger of myrFMN to activate luciferases[1].

Some photobacterial strains carry an additional gene, termed luxF, which produces the LuxF protein, whose function and influence on bacterial bioluminescence is still uncertain. The LuxF protein binds the flavin derivative 6-(3′-(R)-myristyl)-flavin mononucleotide (myrFMN), which is generated as a side product in the luciferase-catalyzed reaction. LuxF protein is important to prevent inhibition of the luciferase by myrFMN. Light emission increases in the presence of luxF independent of natural occurrence[2].

Usage and Biology

In our luminescence circuit,LuxF is controlled by Pompc,which accecpts osmotic regulation.In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane,phosphorylates the transcription factor OmpR.Phosphorylated OmpR binds the ompC promoter and activates the transcription.

Reference

[1]Tinikul, Ruchanok et al. “Bacterial luciferase: Molecular mechanisms and applications.” The Enzymes vol. 47 (2020): 427-455. doi:10.1016/bs.enz.2020.06.001

[2]Brodl, E. et al., The impact of LuxF on light intensity in bacterial bioluminescence. J Photochem Photobiol B 207 111881 (2020).

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 76
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 81
  Illegal PstI site found at 76
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 76
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 76
  Illegal NgoMIV site found at 439
  Illegal NgoMIV site found at 478
  Illegal NgoMIV site found at 522
  Illegal AgeI site found at 220
  
• 1000
  COMPATIBLE WITH RFC[1000]