Part:BBa_K5210005

LuxE

LuxE derives from Photorhabdus like Vibrio natriegens.It synthesises the substrate R-CO-AMP using R-COOH[1].

The fundamental enzymes required for bacterial luminescence are encoded by a single operon, luxCDABE, which is found in all species of luminous bacteria.LuxCDABE operon encodes both luciferase (a heterodimer of LuxA and LuxB) and enzymes required for the production of its substrate tetradecanal (LuxC, LuxD and LuxE).LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal.

Usage and Biology

In our luminescence circuit,LuxE is controlled by Pompc,which accecpts osmotic regulation.In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane,phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the ompC promoter and activates the transcription.

Reference

[1]Tinikul, Ruchanok et al. “Bacterial luciferase: Molecular mechanisms and applications.” The Enzymes vol. 47 (2020): 427-455. doi:10.1016/bs.enz.2020.06.001 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 97