Part:BBa_K5210004

LuxD

Light production in luminous bacteria results from an enzymatic reaction of a substrate catalyzed by a bacterial luciferase[1]. Bacterial luciferase oxidizes reduced flavin mononucleotide (FMNH2) and a long-chain fatty aldehyde (RCHO) to yield flavin mononucleotide (FMN) and the corresponding long-chain fatty acid (RCOOH)[2]. This reaction concomitantly generates blue-green light with a peak wavelength around 490 nm.

The fundamental enzymes required for bacterial luminescence are encoded by a single operon, luxCDABE, which is found in all species of luminous bacteria.LuxCDABE operon encodes both luciferase (a heterodimer of LuxA and LuxB) and enzymes required for the production of its substrate tetradecanal (LuxC, LuxD and LuxE).LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal.LuxD synthesises R-CHO using R-CO-AMP and forms the substrate along with LuxC and LuxE.

Usage and Biology

In our luminescence circuit,LuxD is controlled by Pompc,which accecpts osmotic regulation.In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane,phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the ompC promoter and activates the transcription.

Reference

[1]Hastings JW, Nealson KH. Bacterial bioluminescence. Ann. Rev. Microbiol. 1977;31:549–595. doi: 10.1146/annurev.mi.31.100177.003001

[2]Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol. Rev. 1991;55:123–142. doi: 10.1128/mr.55.1.123-142.1991 Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 128
  Illegal PstI site found at 785
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 128
  Illegal PstI site found at 785
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 128
  Illegal PstI site found at 785
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 128
  Illegal PstI site found at 785
  
• 1000
  COMPATIBLE WITH RFC[1000]