Part:BBa_K5210003
LuxC
LuxCDABE operon from Photorhabdus like Vibrio natriegens encodes both luciferase (a heterodimer of LuxA and LuxB) and enzymes required for the production of its substrate tetradecanal (LuxC, LuxD and LuxE)[1].LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal.LuxC along with Lux D and Lux E engineer alternative routes for fatty aldehyde production.
Usage and Biology
In our luminescence circuit,LuxC is controlled by Pompc,which accecpts osmotic regulation.In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane,phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the ompC promoter and activates the transcription. High osmotic pressure environments activate LuxC expression and sustained substrate accumulation for luminescence.
Reference
[1]Craney, Arryn et al. “A synthetic luxCDABE gene cluster optimized for expression in high-GC bacteria.” Nucleic acids research vol. 35,6 (2007): e46. doi:10.1093/nar/gkm086 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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