Part:BBa_K5210001

LuxA

The LuxA derives from Photorhabdus like Vibrio natriegens.It encodes the α-subunit of the heterodimeric enzyme LuxAB and is responsible for the light-emitting reaction[1].

Under the action of molecular oxygen and lucifase,the reduced flavin mononotide (FMNH2) and long chain fatty aldehyde (RCHO) are oxidized to FMN and long chain fatty acid (RCOOH), and blue-green light with a wavelength of 490nm is emitted.Bacterial luciferase consists of a heterodimer containing an α subunit (molecular weight 42kDa) and a β subunit (molecular weight 37kDa),and luciferase is active only when the two subunits coexist[2].

Usage and Biology

In our luminescence circuit, LuxA is controlled by PumuDC and Plam which is blocked by deterrent CI protein. During the daytime, PumuDC can be actived by UV radiation in the sunlight. At night, LuxA and luxB express simultaneously, so LuxA can bind with luxB and form luxAB. LuxAB is a luciferase which can utilize the aldehyde made by LuxCDE to produce blue-green light.

Reference

[1]Belas, R et al. “Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi.” Science (New York, N.Y.) vol. 218,4574 (1982): 791-3. doi:10.1126/science.10636771

[2]Kaku, Tomomi et al. “Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer.” Scientific reports vol. 11,1 14994. 22 Jul. 2021, doi:10.1038/s41598-021-94551-

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 154
  
• 1000
  COMPATIBLE WITH RFC[1000]