Coding

Part:BBa_K5207005

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)


CYP76AH22

It is a member of the CYP76AH subfamily. SmCYP76AH22 is homologous to CYP76AH3 and also produces 11-hydroxyferriol. Next, three successive C20 oxidations catalyzed by CYP76AK6-8 converted 11-hydroxyferrocenol to carnosic acid, which in turn spontaneously oxidized to carnosol. We inserted it into pYTK001 by method above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis

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