Coding

Part:BBa_K5207001

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)


SmKSL1

This gene takes charge of the synthesis of Kaurene synthase like, which is A key enzyme in tanshinone synthesis downstream of the MEP pathway that catalyzes the production of miltiradiene. We employed the same methodology for inserting SmGGPPs to integrate SmKSL1 into the pYTK001 vector.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis

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