DNA

Part:BBa_K5204004

Designed by: Yao Chen   Group: iGEM24_NPU-CHINA   (2024-09-26)


Transcription sequence of P. putida KT2440

It will connected with the strong promoter to construct a engineered Ccm, which will be then transform into plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 97
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 97
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 97
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 97
    Illegal NgoMIV site found at 85
    Illegal NgoMIV site found at 318
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 1269
    Illegal NgoMIV site found at 1429
    Illegal NgoMIV site found at 1536
    Illegal AgeI site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None