Coding

Part:BBa_K5203003

Designed by: Guljahon Mustafokulova   Group: iGEM24_NYC-Empire-State   (2024-10-02)

Lysostaphin

Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. It was first isolated from a culture of Staphylococcus simulans by Schindler and Schuhardt in 1964 (Schindler et al., 1964). The 2024 iGEM team NYC-Empire-State integrated epidermin into a bacteriocin operon. This operon consisted of three other antimicrobial peptides, including nisin, pep5, and epidermin, all separated by the AgrC-AgrA linker and activated by the P2 promoter.

References: SCHINDLER, C. A., & SCHUHARDT, V. T. (1964). LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proceedings of the National Academy of Sciences of the United States of America, 51(3), 414–421. https://doi.org/10.1073/pnas.51.3.414


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None