Coding

Part:BBa_K5199003

Designed by: J. Jordan Steel   Group: iGEM24_USAFA   (2024-09-27)


Truncated Silicatein alpha from Suberites domuncula

This is an engineered and truncated version of Suberites domuncula's silicatein alpha gene that has been codon optimized for E coli. It was engineered and gifted from collaboraters at the University of Virginia. The silicatein enzyme is able to be expressed in E. coli and can functionally precipitate silica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 45


[edit]
Categories
Parameters
None