Composite

Part:BBa_K5198105

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)


IFN-NanoLuc
NanoLuciferase (BBa_K1734004 ) with IFN tag (BBa_K4646001 ) . This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS (Figure 1).

Description of the image

Figure 1. Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 527


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