Composite

Part:BBa_K5198102:Design

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)


IFN_SEAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal NgoMIV site found at 1558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 919
    Illegal BsaI.rc site found at 1408


Design Notes

The sequence was designed for extracellular secretion in mammalian cells.


Source

Refer to sources of parts BBa_K4646003, BBa_K1470004.

References