Composite

Part:BBa_K5198101:Design

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)


Igk_SEAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 1534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 895
    Illegal BsaI.rc site found at 1384


Design Notes

The sequence was designed for extracellular secretion in mammalian cells.


Source

Refer to sources of parts BBa_K4646003, BBa_K1470004.

References