Plasmid

Part:BBa_K5196000

Designed by: Sriram Garapati   Group: iGEM24_Michigan   (2024-09-27)


pJN105-NicA2 Plasmid

This is the pJN105-NicA2 plasmid obtained from the Bardwell lab at the University of Michigan. It was used in our experimentation as the backbone for our THFMO construct to be inserted into, following the excision of the nicotine degradation gene (NicA2) it contained and replacing it with the THFMO construct (made of four components, thmA, thmD, thmB, and thmC). It contains a gentamicin resistance gene and an araBAD (arabinose-inducible) promoter.

Sequence and Features:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2782
    Illegal XbaI site found at 4251
    Illegal PstI site found at 3021
    Illegal PstI site found at 3135
    Illegal PstI site found at 3417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2782
    Illegal NheI site found at 2776
    Illegal PstI site found at 3021
    Illegal PstI site found at 3135
    Illegal PstI site found at 3417
    Illegal NotI site found at 4257
    Illegal NotI site found at 7170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2782
    Illegal BglII site found at 476
    Illegal BamHI site found at 2715
    Illegal XhoI site found at 1462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2782
    Illegal XbaI site found at 4251
    Illegal PstI site found at 3021
    Illegal PstI site found at 3135
    Illegal PstI site found at 3417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2782
    Illegal XbaI site found at 4251
    Illegal PstI site found at 3021
    Illegal PstI site found at 3135
    Illegal PstI site found at 3417
    Illegal NgoMIV site found at 4898
    Illegal AgeI site found at 2550
    Illegal AgeI site found at 4738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3462
    Illegal SapI site found at 2532


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