Part:BBa_K5195004
mNeonGreen (for DUX4-DBD)
Description: Base pairs: 708 Origin: bilaterian Branchiostoma lanceolatum, variant of from Aequorea victoria backbone, Properties: Enhance signal intensity as compared to GFP, with 506 nm excitation and 517 nm emittance, low acid sensitivity [1]
Usage and Biology:
mNeonGreen is a commonly utilized tool and one of many fluorophores that are seen to contrast cell expression of genes. It does not alter the intracellular location of the fusion protein, nor does it require a cofactor to produce its pigment, making it particularly useful for tagging genes of interest. [2]
Its enhanced brightness is relatively more intense compared to eGFP, and it maintains an 11-stranded barrel-shaped fluorescent protein in its crystal structure. No aromatic groups in a coplanar orientation exist in the structure of the mNeonGreen protein, which can be a basis for why its emission is shifted rightmost on relative to eGFP. [2]
Our team utilized mNeonGreen as a visual marker for tracking migration differences of our plasmids and was fused to our inducible DUX4-DBD for contrasting results. To ensure the construct was not constitutive, we added a P2A linker to its build. With this plasmid, the main coding sequence of mNG-P2A-DBD was later fused with an additional KRAB repressor domain to further enhance the competition between fusion to DUX4 binding sites.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 161
Illegal SapI.rc site found at 133
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