Composite
cex_cenA

Part:BBa_K5193002:Design

Designed by: CHIEN-YUEH LIU   Group: iGEM24_PuiChing-Macau   (2024-09-15)


exoglucanase and endoglucanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 524
    Illegal NotI site found at 1501
    Illegal NotI site found at 2645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2558
    Illegal BamHI site found at 1694
    Illegal XhoI site found at 2056
    Illegal XhoI site found at 2305
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 530
    Illegal NgoMIV site found at 1032
    Illegal NgoMIV site found at 1836
    Illegal NgoMIV site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1740
    Illegal BsaI.rc site found at 577
    Illegal SapI.rc site found at 660


Design Notes

We used pETDuet backbone for this plasmid and inserted the two genes into the Multiple Cloning Site (MCS) blocks (with separate, two P7 promoters).


Source

Source: existing iGEM part BBa_K118022 and BBa_K118023.

References