Coding

Part:BBa_K5191002

Designed by: Judy Li   Group: iGEM24_NNHS   (2024-09-27)


pMagHigh1-C-split RNAP fusion protein

The pMagHigh1-C-terminal RNAP fusion protein is a tool in gene editing that combines pMagHigh1, a photoresponsive protein, with C-terminal RNA polymerase (RNAP). The pMagHigh1 component can combine with nMagHigh1 under blue light, providing a means to control the activity of the fusion protein spatially and temporally. This fusion allows for controllable RNA synthesize by utilizing blue light to activate or deactivate the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None