Coding
Part:BBa_K5191001
Designed by: Zhixing Sun Group: iGEM24_NNHS (2024-09-27)
rAPOBEC1-nMagHigh1-N-terminal RNAP fusion protein
The rAPOBEC1-nMagHigh1-N-terminal RNAP fusion protein is a tool in gene editing that combines the cytidine deaminase rAPOBEC1 with nMagHigh1, a photoresponsive protein, and N-terminal RNA polymerase (RNAP). The nMagHigh1 component can combine with pMagHigh1 under blue light, providing a means to control the activity of the fusion protein spatially and temporally. This fusion allows for RNA synthesize with precise DNA editing by enabling rAPOBEC1 to convert cytidine (C) to thymine (T) in DNA under blue light. By utilizing light to activate or deactivate the protein, researchers can achieve targeted modifications in DNA, enhancing the specificity of gene editing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |