Coding

Part:BBa_K5191001

Designed by: Zhixing Sun   Group: iGEM24_NNHS   (2024-09-27)


rAPOBEC1-nMagHigh1-N-terminal RNAP fusion protein

The rAPOBEC1-nMagHigh1-N-terminal RNAP fusion protein is a tool in gene editing that combines the cytidine deaminase rAPOBEC1 with nMagHigh1, a photoresponsive protein, and N-terminal RNA polymerase (RNAP). The nMagHigh1 component can combine with pMagHigh1 under blue light, providing a means to control the activity of the fusion protein spatially and temporally. This fusion allows for RNA synthesize with precise DNA editing by enabling rAPOBEC1 to convert cytidine (C) to thymine (T) in DNA under blue light. By utilizing light to activate or deactivate the protein, researchers can achieve targeted modifications in DNA, enhancing the specificity of gene editing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None