Part:BBa_K5184069
G1M5-Cs1A-his
In order to eliminate T.urticae of infested cultivations, spider venom peptide G1M5-Cs1A-his is incorporated in our project with pesticidal means. Cs1a is a small cysteine-rich venom peptide derived from Calommmata signata. Utilized as predatory toxin in nature, it carries the ability to cause paralysis and death in susceptible subjects by blocking voltage-gated calcium channels of them. Having the voltage-gated calcium channels playing a fundamental role in the neuronal system, targets experience fast immobilization after envenomation, thus it serves as an effective pesticide. Due to the cysteine-rich nature of Cs1A, expression strategy that exterminates inclusion bodies is required, thus a G1M5 secretion system is engineered with Cs1A to achieve this. Considering future pesticidal control, G1M5-Cs1A-his can provide future iGEM teams that dedicate in exterminating other destructive pests more choices of sustainable pesticide that could be expressed correctly in Escherichia Coli.
Usage and Biology
Paralysis and mortal effects of susceptible subjects are achieved via Cs1A inhibition on both high-voltage-activated (HVA) Cav channels and low-voltage-activated (LVA) Cav channels of susceptible targets by blocking the channels directly, resulting in impediment of fundamental nervous system responses in neuronal, muscular, and cardiac functions. (Specific site of inhibition depends on the concentration of neurotoxin). G1M5 is the mutated, less hydrophobic version of the secretion signal peptide of the G1 cyclomaltodextrin glucanotransferase (CGtase) of Bacillus sp., which allows the extracellular secretion of the bacterial enzyme. Conduction of proteins attached by G1M5 out of the cytosol is achieved by the Sec pathway, a very common secretion system seen in all three major domains of life: arachaea, prokaryote, and eukaryotes. Once the signal peptide, in this case G1M5 is synthesized, the protein chaperon SecB binds to the preprotein (that is attached to G1M5), and transfers the preprotein to the protein translocase SecA, of which binds to the membrane bound protein conducting channel SecYEG. Once bound to the membrane, SecA binds to a molecule of ATP, of which is hydrolyzed to conduct the protein through heterotrimer complex of SecYEG. A membrane bound SPaseI then, once enough of the preprotein had been conducted through the channel, will remove the SP and allow the preprotein to fold properly into the correct protein. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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