Composite

Part:BBa_K5181019:Design

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-09-26)


rhaBAD_RBS_P450-CPR-fusion protein(BM linker)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2025
    Illegal EcoRI site found at 3254
    Illegal PstI site found at 2696
    Illegal PstI site found at 4799
    Illegal PstI site found at 5078
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2025
    Illegal EcoRI site found at 3254
    Illegal NheI site found at 316
    Illegal PstI site found at 2696
    Illegal PstI site found at 4799
    Illegal PstI site found at 5078
    Illegal NotI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2025
    Illegal EcoRI site found at 3254
    Illegal BglII site found at 1345
    Illegal BglII site found at 2065
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2025
    Illegal EcoRI site found at 3254
    Illegal PstI site found at 2696
    Illegal PstI site found at 4799
    Illegal PstI site found at 5078
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2025
    Illegal EcoRI site found at 3254
    Illegal PstI site found at 2696
    Illegal PstI site found at 4799
    Illegal PstI site found at 5078
    Illegal AgeI site found at 1613
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

later


Source

later

References