Coding

Part:BBa_K5181016:Design

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-09-11)


P450_CPR fusion protein(BM linker)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2665
    Illegal PstI site found at 2944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 370
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2665
    Illegal PstI site found at 2944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2328
    Illegal BamHI site found at 3364
    Illegal XhoI site found at 1306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2665
    Illegal PstI site found at 2944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2665
    Illegal PstI site found at 2944
    Illegal NgoMIV site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The C terminal of truncated P450 and N terminal of truncated CPR1 was joined by BM linker to design Fusion protein.


Source

The part is composed of 3 basic parts (BBa_K5181001, BBa_K5181003, BBa_K5181012) and the gene sequences are souced from NCBI and further truncated.

References