Protein_Domain

Part:BBa_K5181011:Design

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-09-01)


GST_linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is flanked by two ScaI sites, which code for Threonine-Serine residues. This design makes it a replaceable linker sequence.


Source

Leonard E, Koffas MAG 2007. Engineering of Artificial Plant Cytochrome P450 Enzymes for Synthesis of Isoflavones by Escherichia coli. Appl Environ Microbiol 73:. https://doi.org/10.1128/AEM.01411-07

References