Intermediate

Part:BBa_K5181009

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-08-29)


tet promoter with RBS

General information: This part is a self-sufficient tetracyclin promoter with its Supporting gene tetR and RBS(Ribosome Binding site). This can be reconstituted with a P450_CPR insert to form a functional composite part that can be ligated with pSEVA backbones. The annotation of this part is as follows:

Part Image


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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