Intermediate

Part:BBa_K5181006:Design

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-16)


Self sufficient araBAD promoter with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 991


Design Notes

30 bp from 5' end matches with pSEVA MCS architecture and thus can be Gibson cloned or added via restriction digestion



Source

araBAD promoter along with RBS sequence was obtained from pBbE8k-fcs plasmid from addgene.

References

araBAD promoter (https://www.addgene.org/83068/sequences/)