Coding

Part:BBa_K5181003

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-13)


CPR_truncated: Sandalwood CPR without transmembrane domain

CPR_truncated(cytochrome P450 reductase ) is the reducing partner of the truncated P450 (BBa_K5181001) without its Transmembrane Domain. The modification was made to make it soluble in the cytosol of P. putida and thus be functional inside it. 147 nucleotides (49) amino acids were removed from the N-terminal Domain to truncate the Transmembrane domain.

The alphafold predicted structure of the protein is:

The transmembrane tendency of wild type and truncated CPR1


This shows that truncation of the transmembrane domain of CPR1 truly reduced the transmembrane tendencies.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None