Part:BBa_K5181002:Design

CPR_native_Sandalwood

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 946
  Illegal PstI site found at 1399
  Illegal PstI site found at 1678
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 946
  Illegal PstI site found at 1399
  Illegal PstI site found at 1678
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 946
  Illegal BglII site found at 1062
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 946
  Illegal PstI site found at 1399
  Illegal PstI site found at 1678
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 946
  Illegal PstI site found at 1399
  Illegal PstI site found at 1678
  Illegal NgoMIV site found at 60
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The gene sequence is codon optimized for Pseudomonas putida using GenScript's Codon optimization tool. Amino acid sequence : MSSSSELWKSIGSALGVSPPPAWAEEWAAVIVTTSAALIVGFVMFMWRRSGEKSKELRPVVALKAAPIEAEEDDGEVDSGKTKVTVFFGTQTGTAEGFAKA LAEEIKARYEKAVVKVVDLDDYAADDDQYGEKLKNETLTFFMVATYGDGEPTDNAARFYKWFTEEKEREAWLQQLTYGIFGLGNRQYEHFNKIAKVLDEQL TEQGAKRLIQVGLGDDDQCIEDDFSAWRELLWPELDQLLRGDDGANSVSTPYTAAVPEYRVVIHDPTITSSEDKSLATANGAALFDIHHPCRVKVAVQREL HKADSDRSCIHLEFDISGTGLMYETGDHVGVYAENCVETVEEAGKLLGQPLDLLFSVHTDKDDGTSLESSLPPPFPGPCTLRTALFQYADLLNPPRKAALV ALAAHAVEPSEADRLKFLSSPQGKDEYAKWVVGSQRSLLEVMAEFPSIKVPLGVFFAAVAPRLQPRYYSISSSPRFSSDRVHVTCALVYGPSPTGRIHRGV CSTWMKNAVPLEESRECSWAPIFIRTSNFKLPANPSTPVIMVGPGTGLAPFRGFLQERMALLEGSAQLGPALLFFGCRNRRMDFIYEDELNNFVEQGVISE LIVAFSRDGPTKEYVQHKMMDKAAYIWSLISQGAYLYVCGDAKGMARDVHRTLHTLVQQQESVDSSKAESIVKKLQMDGRYLRDVWHHHHHH

It has a 6X his-tag at its end and can be purified by a Ni-NTA column.

Source

Santalum album, NCBI(KC842187.1)