Coding

Part:BBa_K5181002

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-08-13)


CPR_native_Sandalwood

This part contains a Cytochrome P450 reductase (CPR) gene sequence obtained from the Santalum album (Sandalwood tree) genome. This CPR enzyme is a reductase partner for Cytochrome P450 monooxygenase (BBa_K5181000). It replenishes and shuttles the FADH2 required for its functioning. The protein coding region was obtained from NCBI(KC842187.1) and was codon optimized for Pseudomonas putida.

The Alphafoldpredicted structure of the protein is:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 946
    Illegal PstI site found at 1399
    Illegal PstI site found at 1678
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 946
    Illegal PstI site found at 1399
    Illegal PstI site found at 1678
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 946
    Illegal BglII site found at 1062
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 946
    Illegal PstI site found at 1399
    Illegal PstI site found at 1678
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 946
    Illegal PstI site found at 1399
    Illegal PstI site found at 1678
    Illegal NgoMIV site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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