Coding

Part:BBa_K5181000

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-08-07)


P450_native_Santalum album

This part contains a cytochrome P450 mono-oxygenase CYP736A167 gene sequence in the Santalum album (Sandalwood tree). This P450 converts alpha santalene, beta santalene, epi-beta santalene, and alpha-exo-bergamotene to their respective 'ol' forms by oxidizing them. It is a transmembrane protein and requires a Cytochrome P450 reductase (CPR) to replenish and shuttle the FADH2 required for its functioning. The protein coding region was obtained from NCBI and was codon optimized for Pseudomonas putida

The Alphafoldpredicted structure of the protein is:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 298
    Illegal PstI site found at 976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 298
    Illegal PstI site found at 976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 298
    Illegal PstI site found at 976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 298
    Illegal PstI site found at 976
    Illegal NgoMIV site found at 427
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None