Regulatory
sulAp
Part:BBa_K518010:Experience
Designed by: Masato Ohgishi Group: iGEM11_UT-Tokyo (2011-09-27)
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how you used this part and how it worked out.
Applications of BBa_K518010
User Reviews
UNIQ8c104ae01d6e3068-partinfo-00000000-QINU
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Cooper Union iGEM 2014 |
We used this part to design a composite part, BBa_K1354000, with this promoter and a phage activator, BBa_I746352. This phage activator turned on a phage promoter with a GFP reporter composite part, BBa_I746321. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below. |
UNIQ8c104ae01d6e3068-partinfo-00000005-QINU