Terminator

Part:BBa_K5179301:Design

Designed by: Caden Sanko   Group: iGEM24_William-and-Mary   (2024-10-01)


P4 T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 46
    Illegal BsaI.rc site found at 40


Design Notes

None


Source

The T1 terminator is from satellite bacteriophage P4.

References

Forti, F., Dragoni, I., Briani, F., Dehò, G., & Ghisotti, D. (2002). Characterization of the small antisense CI RNA that regulates bacteriophage P4 immunity. Journal of molecular biology, 315(4), 541–549.

Sabbattini, P., Forti, F., Ghisotti, D., & Dehò, G. (1995). Control of transcription termination by an RNA factor in bacteriophage P4 immunity: identification of the target sites. Journal of bacteriology, 177(6), 1425–1434.