Translational_Unit

Part:BBa_K5179214:Design

Designed by: Rachel McCarley   Group: iGEM24_William-and-Mary   (2024-09-30)


L5 Δ homolog


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 724
    Illegal XhoI site found at 175
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
    Illegal NgoMIV site found at 955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 378
    Illegal BsaI.rc site found at 178


Design Notes

None


Source

Made from concatenated protein domains of phage L5 and HerbertWM. The DNA used to assemble this part was obtained from IDT (Integrated DNA Technologies).

References