Translational_Unit

Part:BBa_K5179214

Designed by: Rachel McCarley   Group: iGEM24_William-and-Mary   (2024-09-30)


L5 Δ homolog

This is a chimeric tail protein containing segments of the Gp6 gene from Mycobacteriophage L5, with a homologous region of the sequence substituted out with that of Mycobacteriophage HerbertWM’s Gp3 gene. We used this part in our project to attempt host-range expansion of the Mycobacteriophage HerbertWM to include Mycobacterium smegmatis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 724
    Illegal XhoI site found at 175
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
    Illegal PstI site found at 736
    Illegal NgoMIV site found at 955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 378
    Illegal BsaI.rc site found at 178


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Categories
Parameters
None