Translational_Unit

Part:BBa_K5179213

Designed by: Rachel McCarley   Group: iGEM24_William-and-Mary   (2024-09-30)


WT L5 Gp6

Gene Product 6, or Gp6, is a wild-type tail protein gene from the Mycobacteriophage L5. We used this part in our project to attempt host-range expansion of the Mycobacteriophage HerbertWM to include Mycobacterium smegmatis.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 706
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 706
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 694
    Illegal XhoI site found at 61
    Illegal XhoI site found at 145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 706
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 706
    Illegal NgoMIV site found at 925
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 348
    Illegal BsaI.rc site found at 148


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