Translational_Unit

Part:BBa_K5179212

Designed by: Rachel McCarley   Group: iGEM24_William-and-Mary   (2024-09-30)


HerbertWM Gp3 Mutant

This is a chimeric tail protein containing the RBS and initial homologous region associated with gene Gp3 of the Mycobacteriophage HerbertWM, followed by the remainder of the corresponding gene from Mycobacteriophage L5 Gp6. We used this part in our project to attempt host-range expansion of the Mycobacteriophage HerbertWM to include Mycobacterium smegmatis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
    Illegal XhoI site found at 186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 389
    Illegal BsaI.rc site found at 189


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Categories
Parameters
None