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Part:BBa_K5179201

Designed by: Rebecca Zheleznyak   Group: iGEM24_William-and-Mary   (2024-09-30)


Mycobacteria Phage Satellite ChrisB1

The mycobacteria phage ChrisB1 is a satellite phage that infects a strain of bacteria Mycobacterium aichiense. This strain of bacteria has the prophage HerbertWM, which expresses strong anti-phage defense mechanisms. The phage satellite hijacks the mechanism of HerbertWM in order to package itself and lyse the cell. While doing so, it also initiates the excision of HerbertWM, meaning the lysate contains both the phage satellite and HerbertWM.

This unique mechanism makes it a valuable part in overcoming prophage immunity in M. aichiense. We use this in our project both in vitro and in situ in a simulated soil environment.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2241
    Illegal SpeI site found at 6103
    Illegal PstI site found at 1918
    Illegal PstI site found at 3616
    Illegal PstI site found at 8501
    Illegal PstI site found at 8872
    Illegal PstI site found at 9691
    Illegal PstI site found at 9817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 6103
    Illegal PstI site found at 1918
    Illegal PstI site found at 3616
    Illegal PstI site found at 8501
    Illegal PstI site found at 8872
    Illegal PstI site found at 9691
    Illegal PstI site found at 9817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4072
    Illegal BglII site found at 4869
    Illegal BglII site found at 5077
    Illegal BamHI site found at 3574
    Illegal BamHI site found at 7629
    Illegal BamHI site found at 8574
    Illegal XhoI site found at 5733
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2241
    Illegal SpeI site found at 6103
    Illegal PstI site found at 1918
    Illegal PstI site found at 3616
    Illegal PstI site found at 8501
    Illegal PstI site found at 8872
    Illegal PstI site found at 9691
    Illegal PstI site found at 9817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2241
    Illegal SpeI site found at 6103
    Illegal PstI site found at 1918
    Illegal PstI site found at 3616
    Illegal PstI site found at 8501
    Illegal PstI site found at 8872
    Illegal PstI site found at 9691
    Illegal PstI site found at 9817
    Illegal NgoMIV site found at 7498
    Illegal NgoMIV site found at 9829
    Illegal AgeI site found at 3
    Illegal AgeI site found at 2469
    Illegal AgeI site found at 2560
    Illegal AgeI site found at 9846
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 350
    Illegal BsaI site found at 2522
    Illegal BsaI site found at 2657
    Illegal BsaI site found at 6468
    Illegal BsaI site found at 9366
    Illegal BsaI site found at 9432
    Illegal BsaI.rc site found at 1018
    Illegal BsaI.rc site found at 2639
    Illegal BsaI.rc site found at 3750
    Illegal BsaI.rc site found at 5793
    Illegal BsaI.rc site found at 6183
    Illegal BsaI.rc site found at 6478
    Illegal BsaI.rc site found at 9739
    Illegal SapI site found at 1583
    Illegal SapI site found at 3513
    Illegal SapI.rc site found at 443
    Illegal SapI.rc site found at 1517
    Illegal SapI.rc site found at 9078
    Illegal SapI.rc site found at 10338


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