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Part:BBa_K5179200

Designed by: Rebecca Zheleznyak   Group: iGEM24_William-and-Mary   (2024-09-30)


Mycobacteria Phage Satellite Anya

The mycobacteria phage Anya is a satellite phage that infects a strain of bacteria Mycobacterium aichiense. This strain of bacteria has the prophage HerbertWM, which expresses strong anti-phage defense mechanisms. The phage satellite hijacks the mechanism of HerbertWM in order to package itself and lyse the cell. While doing so, it also initiates the excision of HerbertWM, meaning the lysate contains both the phage satellite and HerbertWM.

This unique mechanism makes it a valuable part in overcoming prophage immunity in M. aichiense. We use this in our project both in vitro and in situ in a simulated soil environment.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 27
    Illegal SpeI site found at 3889
    Illegal PstI site found at 1402
    Illegal PstI site found at 6287
    Illegal PstI site found at 6658
    Illegal PstI site found at 7477
    Illegal PstI site found at 7603
    Illegal PstI site found at 10576
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3889
    Illegal PstI site found at 1402
    Illegal PstI site found at 6287
    Illegal PstI site found at 6658
    Illegal PstI site found at 7477
    Illegal PstI site found at 7603
    Illegal PstI site found at 10576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1858
    Illegal BglII site found at 2655
    Illegal BglII site found at 2863
    Illegal BamHI site found at 1360
    Illegal BamHI site found at 5415
    Illegal BamHI site found at 6360
    Illegal XhoI site found at 3519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 27
    Illegal SpeI site found at 3889
    Illegal PstI site found at 1402
    Illegal PstI site found at 6287
    Illegal PstI site found at 6658
    Illegal PstI site found at 7477
    Illegal PstI site found at 7603
    Illegal PstI site found at 10576
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 27
    Illegal SpeI site found at 3889
    Illegal PstI site found at 1402
    Illegal PstI site found at 6287
    Illegal PstI site found at 6658
    Illegal PstI site found at 7477
    Illegal PstI site found at 7603
    Illegal PstI site found at 10576
    Illegal NgoMIV site found at 5284
    Illegal NgoMIV site found at 7615
    Illegal AgeI site found at 255
    Illegal AgeI site found at 346
    Illegal AgeI site found at 7632
    Illegal AgeI site found at 8661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 308
    Illegal BsaI site found at 443
    Illegal BsaI site found at 4254
    Illegal BsaI site found at 7152
    Illegal BsaI site found at 7218
    Illegal BsaI site found at 9008
    Illegal BsaI.rc site found at 425
    Illegal BsaI.rc site found at 1536
    Illegal BsaI.rc site found at 3579
    Illegal BsaI.rc site found at 3969
    Illegal BsaI.rc site found at 4264
    Illegal BsaI.rc site found at 7525
    Illegal BsaI.rc site found at 9676
    Illegal SapI site found at 1299
    Illegal SapI site found at 10241
    Illegal SapI.rc site found at 6864
    Illegal SapI.rc site found at 8124
    Illegal SapI.rc site found at 9101
    Illegal SapI.rc site found at 10175


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